Detection of transgenic herbicide resistant rapeseed plants in Kiev region

  • Б. В. Моргун Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine Ukraine, 03680, Kyiv, Akademika Zabolotnogo str., 148
  • О. В. Степаненко Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine Ukraine, 03680, Kyiv, Akademika Zabolotnogo str., 148
  • А. І. Степаненко Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine Ukraine, 03680, Kyiv, Akademika Zabolotnogo str., 148
  • А. М. Тараненко Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine Ukraine, 03680, Kyiv, Akademika Zabolotnogo str., 148
  • М. В. Кучук Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine Ukraine, 03680, Kyiv, Akademika Zabolotnogo str., 148

Abstract

Aim. Unauthorized entry of transgenic plants on the territory of Ukraine is an important issue that requires detailed analysis and solution. To determine the degree of high quality rapeseed contamination from Kyiv region with transgenetic material there was performed its monitoring. Methods. 270 seed samples were analyzed in physiological experiments. Selected samples were analyzed using molecular genetic methods. Results. One of the samples was found to be resistant to glyphosate herbicide and another to glufosinate ammonium herbicide. The collected samples were examined using molecular genetic techniques that confirmed the presence of transgenes CP4 epsps and bar, respectively responsible for these types of resistance. In one of the samples there was identified the transformation event GT73 of Monsanto responsible for glyphosate resistance. Conclusions. These data suggest the uncontrolled movements of genetically modified canola plants on the territory of Ukraine.

Key words: Brassica napus, glyphosate, herbicide Ouragan Forte 500 SL в.р.к. Syngenta, herbicide Basta Bayer CropScience, GMO.