Detection of wheat-rye translocations by means of DNA markers and electrophoresis of proteins
Aim. Aim is to identify opportunities to use molecular genetic labeling of genes and electrophoresis of protein gliadin fractions for detection of 1AL.1RS and 1VL.1RS wheat-rye translocations. Methods. PCR analysis of wheat DNA, protein electrophoresis in polyacrylamide gels. Results. Wheat-rye translocations in winter wheat were identified. Conclusions. Polymerase chain reaction using specific primers and electrophoresis of protein gliadin fractions are reliable, efficient and complementary methods for identifying wheat-rye translocations.
Keywords: winter soft wheat, wheat-rye translocations, PCR-analysis, DNA-markers, electrophoresis of proteins.