Detection of wheat-rye translocations by means of DNA markers and electrophoresis of proteins

  • А. І. Степаненко Institute of Cell Biology and Genetic Engineering National Academy of Sciences of Ukraine Ukraine, 03680, Kyiv, Akademika Zabolotnogo str., 148
  • О. М. Благодарова Plant Breeding & Genetics Institute – National Center of Seed and Cultivar Investigation Ukraine, 65036, Odessa, Ovidiopolskaya road, 3
  • Б. В. Моргун Institute of Cell Biology and Genetic Engineering National Academy of Sciences of Ukraine Ukraine, 03680, Kyiv, Akademika Zabolotnogo str., 148
  • Т. В. Чугункова Institute of Plant Physiology and Genetics National Academy of Sciences of Ukraine Ukraine, 03022, Kyiv, Vasylkivska str., 31/17
  • О. І. Рибалка Plant Breeding & Genetics Institute – National Center of Seed and Cultivar Investigation Ukraine, 65036, Odessa, Ovidiopolskaya road, 3

Abstract

Aim. Aim is to identify opportunities to use molecular genetic labeling of genes and electrophoresis of protein gliadin fractions for detection of 1AL.1RS and 1VL.1RS wheat-rye translocations. Methods. PCR analysis of wheat DNA, protein electrophoresis in polyacrylamide gels. Results. Wheat-rye translocations in winter wheat were identified. Conclusions. Polymerase chain reaction using specific primers and electrophoresis of protein gliadin fractions are reliable, efficient and complementary methods for identifying wheat-rye translocations.

Keywords: winter soft wheat, wheat-rye translocations, PCR-analysis, DNA-markers, electrophoresis of proteins.