Genomic analysis of the  Antarctic actinomycete Micromonospora endolithica strain AA-459

І. І. Roman; O. M. Gromyko

doi:10.7124/visnyk.utgis.22.1-2.1686

І. І. Roman Ivan Franko National University of Lviv Ukraine, 79005, Lviv, Hrushevskoho St. 4 https://orcid.org/0000-0003-4449-739X
O. M. Gromyko Ivan Franko National University of Lviv Ukraine, 79005, Lviv, Hrushevskoho St. 4 https://orcid.org/0000-0002-8107-0128

DOI: https://doi.org/10.7124/visnyk.utgis.22.1-2.1686

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Keywords: bacteria, actinomycetes, Micromonospora, Аntarctic, genomic analysis

Abstract

Aim. To investigate the genome annotation of M. endolithica AA-459 for the presence of unique genes and their combinations, as well as to assess the production potential of the strain by analysing secondary metabolism clusters. Methods. Genome annotation was performed using the RAST tool, and the search for secondary metabolism clusters was performed using the AntiSMASH tool. Results. Genome annotation identified 6,593 coding regions, including 9 rRNA and 67 tRNA genes. Functional characterization revealed genes spanning 24 subsystems. Protein metabolism was represented by 235 genes, carbohydrate metabolism involved 266 genes, 281 genes involved in amino acid metabolism, 99 genes associated with lipid and fatty acid metabolism. AntiSMASH analysis identified 17 potential secondary metabolite clusters in the genome of M. endolithica AA-459. Notably, two clusters showed high homology to those of known compounds. Conclusions. The genome analysis of M. endolithica AA-459 demonstrated the high production potential of the strain, which indicates the importance of the Antarctic region in the context of new compound discovery. However, the number of described compounds from this region remains low, which may be due to the lack of optimal conditions for the expression of the relevant genes. Further research in this area will reveal the biotechnological potential of Antarctic actinomycetes.

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