Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)

  • A. Z. Revutskaya ESC "Institute of Biology and Medicine", Kyiv National Taras Shevchenko University, Ukraine, 01601, Kyiv, Glushkov avenue, 2
  • A. V. Holubenko ESC "Institute of Biology and Medicine", Kyiv National Taras Shevchenko University, Ukraine, 01601, Kyiv, Glushkov avenue, 2
  • N. V. Nuzhyna ESC "Institute of Biology and Medicine", Kyiv National Taras Shevchenko University, Ukraine, 01601, Kyiv, Glushkov avenue, 2
  • H. O. Rudik ESC "Institute of Biology and Medicine", Kyiv National Taras Shevchenko University, Ukraine, 01601, Kyiv, Glushkov avenue, 2
  • N. Yu. Taran Навчально-науковий центр «Інститут біології та медицини», Київський національний університет імені Тараса Шевченка, Україна, 03022, м. Київ, просп. Академіка Глушкова, 2
DOI: https://doi.org/10.7124/visnyk.utgis.17.1.1198

Abstract

Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.
Keywords: Salvia hispanica (Chia), in vitro culture, callus.

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