Характеристика ITS-фрагмента рДНК Rhodotorula mucilaginosa из желудочно-кишечного тракта долгожителя

  • Л. В. Полищук
  • К. С. Ткаченко
  • М. А. Фомина


Aim. The aim of the work was to analyse the primary structure of the ITS region of the ribosomal operon of the red yeast isolate Rhodotorula mucilaginosa S1 from the gastrointestinal tract of healthy centenarian compared to the available homological basidimycetous nucleotide sequences. Methods. In silico comparison of the ITS nucleotide sequences using NCBI server resources (BLAST programs and "Nucleotide" database) with the reference ITS-fragment of red yeast isolate was performed. Results. Almost 90 % of highly homologous to the reference ITS-fragment out of 800 selected sequences represented genera Rhodotorula and Rhodosporidium with the top hits for R. mucilaginosa strains PMM08-1716L, 3942L and 3684L. The most frequent nucleobases mismatches of ITS-fragments of GenBank’s R. mucilaginosa strains compared to the reference one were observed in the positions 541 (T/C) for 85 % of strains, and 129 (T/C) and 158 (G/A) for 26 % of strains. Conclusions. The primary structure of studied ITS region showed 100 % homology to R. mucilaginosa strains also isolated from humans and animals, as well as the most frequently mismatches for 129th, 158th and 541st nucleotides located in the spacers regions of the ribosomal operon.

Keywords: ITS, rDNA, nucleotide sequence, red yeast, human GIT.


Hong J.W., Fomina M., Gadd G.M. F‐RISA fungal clones as potential bioindicators of organic and metal contamination in soil. J. Аppl. Microbiol. 2010. V. 109(2). Р. 415–430. doi: 10.1111/j.1365-2672.2009.04665.x.

Irinyi L., Serena C., Garcia-Hermoso D. International Society of Human and Animal Mycology (ISHAM)-ITS reference DNA barcoding database – the quality controlled standard tool for routine identification of human and animal pathogenic fungi. Medical Mycology. 2015. V. 53(4). P. 313–337. doi: 10.1093/mmy/myv008.

Kurtzman C.P., Fell J.W., Boekhout T. The Yeasts: A Taxonomic Study. Amsterdam – Oxford: Elsevier, 2011. 1053 p.

Nagahama T., Hamamoto M., Nakase T., Takami H., Horikoshi K. Distribution and identification of red yeasts in deep-sea environments around the northwest Pacific Ocean. Antonie Van Leeuwenhoek. 2001. V. 80(2). Р. 101–110. doi: 10.1023/A:1012270503751.

Zimmer C., Platz T., Cadez N., Giffhorn F., Kohring G.W. A cold active (2R,3R)-(-)-di-O-benzoyl-tartrate hydrolyzing esterase from Rhodotorula mucilaginosa. Appl. Microbiol. Biotechnol. 2006. V. 73(1). Р. 132–140. doi: 10.1007/s00253-006-0463-x.

Bab'eva I.P., Golubev V.I. Metody vydeleniia i identifikatsii drozhzhey. Moskva: ‘Pishchevaia promyshlennost’, 1979. 118 p. [in Russian]

Kvasnikov E.I., Vaskivniuk V.T., Sudenko V.I., Grinberg T.A. Karotin-sinteziruiushchie drozhzhi. Kyiv: Naukova dumka, 1980. 171 p. [in Russian]

Fomina M.A., Polishchuk L.V., Tkachenko K.S., Hong J.W., Zelena L.B., Ianieva O.D., Pidgorskyi V.S. Complex identification of red yeast isolate from gastrointestinal tract of Hucul long-liver (Carpathians, Ukraine). Microbiol. Zhurnal. 2016. V. 78(5). P. 2–11.

Buzzini P., Innocenti M., Turchetti B., Libkind D., van Broock M., Mulinacci N. Carotenoid profiles of yeasts belonging to the genera Rhodotorula, Rhodosporidium, Sporobolomyces and Sporidiobolus. Can. J. Microbiol. 2007. V. 53(8). Р. 1024–1031. doi: 10.1139/W07-068.