Биологические эффекты наночастиц селена и cеленита натрия на клеточные культуры многоколосника морщинистого
Aims. Recently search of substances replacing toxic selenites is conducted. The Se nanoparticles and sodium selenite influence on physiological and biochemical parameters of A. rugosa callus tissues were investigated. Methods. Accumulation of Se in callus tissues were measured by nuclear and issue spectrometry. Рrotein content and peroxidase activity were measured by specific spectrometric methods. Results. It was found that A. rugosa callus tissues possessed the expressed ability to Se accumulation. Sodium selenite was more bioavailable for A. rugosa cells than Se nanoparticles, however it was toxic in investigating concentration (10 and 50 mg/l) and caused callus death. Selenium in A. rugosa callus tissues stimulated biosynthesis of protein and modified peroxidase activity. Conclusions. Оnly Se nanoparticles as nontoxical neither for animals, nor for plants apply for a role of the dietary supplement component.
Key words: Agastache rugosa (Fisch. & C.A.Mey.) Kuntze, callus tissues, selenium nanoparticles, sodium selenite.