The photomorphogenetic reaction of pea plants (Pisum sativum L.) on ultraviolet irradiation effect

  • V. V. Zhuk Institute of Cell Biology and Genetic Engineering of Nat. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Academika Zabolotnogo str., 148
  • O. M. Mikhieiev Institute of Cell Biology and Genetic Engineering of Nat. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Academika Zabolotnogo str., 148
  • L. G. Ovsyannikova Institute of Cell Biology and Genetic Engineering of Nat. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Academika Zabolotnogo str., 148


Aim. The comparation of photomorphogenetic response of pea plants in wide dose range of UV-C and UV-B irradiation was studied. Methods. Pea plants (Pisum sativum L.) cultivar Aronis were grown in water culture during 10 days and then were irradiated by UV-B and UV-C irradiation in doses 1, 2, 4, 8 and 15 kJ/m2. The length of shoots and roots and fresh weight of pea plants were measured. Results. It is established that UV-C irradiation caused strong inhibition effect on shoot growth against UV-B in the same doses. It is shown that the dose of 8 kJ/m2 was the threshold of the UV-B and UV-C effect. Dose above 8 kJ/m2 caused growth inhibition. The dose of 2 kJ/m2 caused the hormesis effect. Stem growth inhibition under the UV-C stimulated root growth. Conclusions. Comparative study of UV-B and UV-C irradiation demonstrated that the hormesis effect on plant growth was shown in the same dose of 2 kJ/m2. The inhibition effect of UV-C irradiation was stronger than UV-B in the same doses.

Keywords: UV-B, UV-C, Pisum sativum L., hormesis.


Jenkins G.J. Signal transduction in responses to UV-B radiation. Ann. Rev. Plant Biol. 2009. V. 60. P. 407-431. doi: 10.1146/annurev.arplant.59.032607.092953

Falcone Ferreyra M.L., Casadevall R., D'Andrea L., AbdElgawad H., Beemster G.T., Casati P. AtPDCD5 Plays a role in programmed cell death after UV-B exposure in Arabidopsis. Plant Physiol. 2016. V. 170. P. 2444-2460. doi: 10.1104/pp.16.00033

Singh S., Mishra S., Kumari R., Agrawal S.B. Response of ultraviolet-B and nickel on pigments, metabolites and antioxidants of Pisum sativum L. J. Environ. Biol. 2009. V. 30 (5). P. 677-684.

Singh R., Singh S., Tripathi R., Agrawal S.B. Supplemental UV-B radiation induced changes in growth, pigments and antioxidant pool of bean (Dolichos lablab) under field conditions. J. Environ Biol. 2011. V. 32. P. 139-145.

Morales L.O., Brosche M., Vainonen J., Jenkins G.J., Wargent J.J., Sipari N., Strid A., Lindfors A.V., Tagelbery R., Aphalo P.J. Multiple roles for UVRESISTANCE LOCUS 8 in regulating gene expression and metabolite accumulation in Arabidopsis under solar ultraviolet radiation. Plant Physiol. 2013. V. 161. P. 744-759. doi: 10.1104/pp.112.211375

Hayes S., Sharma A., Fraser D.P., Trevisan M., Cragg-Barber C.K., Tavridov E., Fankhauser Ch., Jenkins G.J., Franklin K.A. UV-B Perceived by the UVR8 photoreceptor inhibits thermomorphogenesis. Curr. Biol. 2017. V. 27. P. 120-123. doi: 10.1016/j.cub.2016.11.004

Shinkle J.R., Atkins A.K., Humphrey E.E., Rodgers Ch.W., Wheeler S.L., Barnes P.W. Growth and morphological responses to different UV waveband (Cucumis sativum) in cucumber and other dicotyledonous seedlings. Physiol. Plant. 2004. V. 120. P. 240-248. doi: 10.1111/j.0031-9317.2004.0237.x

Shinkle J.R., Derickson D.L., Barnes P.W. Comparative physiology of growth responses two UV-B wavebands and UV-C in dim-red-light and white-light-grown cucumber (Cucumis sativus) seedlings: physiological evidence for photoreactivation. Photochem. Photobiol. 2005. V. 81. P. 1069-1074. doi: 10.1562/2005-01-10-RA-411

Shinkle J.R., Edwards M.C., Koenig A., Shaltz A., Barnes P.W. Photomorphogenetic regulation of increases in UV-absorbing pigments in cucumber (Cucumis sativus) and Arabidopsis thaliana seedlings induced by different UV-B and UV-C wavelands. Physiol. Plant. 2010. V. 138. P. 113-121. doi: 10.1111/j.1399-3054.2009.01298.x

Mikhieiev O.M., Zhuk V.V., Ovsiannykova L.H., Hrodzyns'kyy D.M. Hormezysnyy vplyv UF-S oprominennia na pihmentnyy kompleks i antyoksydantni fermenty lystkiv Pisum sativum L.. Dopovidi NAN Ukrainy. 2016. No. 11. P. 99-111. [in Ukrainian]

Urban L., Charles F., de Miranda M.R., Aarrof J. Understanding the physiological effects of UV-C light and exploiting its agronomic potential before and after harvest. Plant Physiol. Biochem. 2016. V. 105. P. 1-11. doi: 10.1016/j.plaphy.2016.04.004