К вопросу идентификации и изучения переноса генетической информации между клетками млекопитающих
Aims. Developing a model system for the study of transfer of genetic information between the cells of mammals is required to obtain reliable and reproducible results. Methods. An embryonic fibroblasts of mouse and white blood cells (WBC) of FVB–Cg–Tg (GFPU)5Nagy/J mouse strain that have expression of enhanced green fluorescent protein (EGFP) were used as objects of our investigation.. After cocultivation for 24 hours of fibroblasts with blood cells WBC were removed from the surface of fibroblasts and fibroblasts were passaged and incubate for 5–7 days more. After these fibroblasts were fixed and analyzed by standard fluorescence microscopy for GFP signals. Results. Microscopy results showed that fibroblasts are present with varying degrees of light intensity in the experimental probes There were a low signal in order to autofluorescence in the control. Conclusion. These results demonstrate the feasibility of reliable transfer of genetic material between cells of WBC and fibroblasts of mice strains with high efficiency and allow us to offer this system as a model to studying the transfer of genetic information between the cells in mammals.
Key words: mice fibroblasts, the WBC of mice, the transfer of genetic information, GFP.