Використання молекулярних маркерів на основі генів відповіді на стрес для аналізу генетичного різноманіття Gentiana lutea L.
The aim of the work was to determine the level of genetic diversity of G. lutea populations
from two mountain ranges (Chornogora, Svydovets) of Ukrainian Carpathians. Methods. Genetic variability of 86 plants from six populations was assessed using CDDP fingerprinting with 6 primers. To evaluate the genetic polymorphism, the percentage of polymorphic loci (P), Shannon’s information index (S), expected heterozygosity (He), gene flow (Nm), Jaccard’s (Dj) and Nei’s (DN) genetic distances were calculated. Results. Using CDDP primers, 120 bands were generated, and 95.8 % of those were polymorphic. The relatively high level of genetic diversity was revealed in G. lutea populations (P = 31 %, S = 0.167, He = 0.112). According to the Bayesian analysis, the populations were clustered in six different groups. Analysis of molecular variance (AMOVA) showed that the differences between populations account for 71 % of total genetic variation, whereas only 29 % of it were resided within populations. Agropopulation from Pozhyzhevska Mountain had low values of genetic polymorphism (He = 0.056, S = 0,089, P = 18.3 %, Dj = 10.9 %). Conclusions. The obtained results of AMOVA indicate significant genetic isolation and differentiation of G. lutea populations. Comparison of G. lutea populations from Chornogora and Svydovets ranges showed that the former have a higher level of genetic heterogeneity. The low level of genetic diversity of Svydovets populations makes it necessary to protect them to ensure their survival.
Keywords: Gentiana lutea L., genetic polymorphism, CDDP-PCR, differentiation of populations.