In planta genetic transformation of bread wheat, using AGLO strain, containing pBi2E with dsRNA-suppressor of ProDH gene

  • С. С. Воронова
  • А. В. Бавол
  • О. В. Дубровна


Aims. The effectiveness of (Triticum aestivum L.) transformation in planta using strain AGLO harboring plasmid pBi2E with dsRNA-suppressor (double sequence RNA-suppressor) of prolinedehydrogenase gene and selective neomycin phosphotransferase II gene (nptII) has been analyzed. Methods. Agrobacterium-mediated genes transfer in planta during wheat pollination. Results. Seeds, RCR-analysis of which confirmed availability pro1 gene exon, of T1- wheat’s plants have been obtained. Conclusions. It has been shown the possibility of stable integration of the transgenes at the wheat’s genome under Agrobacterium-mediated transformation in planta.

Keywords: Triticum aestivum, Agrobacterium-mediated transformation in planta.