Morphological characteristics of processes injury compensation and devices in pathological changes liver CCl4

  • Т. П. Гулько
  • М. В. Драгулян
  • Е. Г. Дерябина
  • В. А. Кордюм
  • М. Ю. Левкив
  • Р. В. Бубнов


Aim. Study of liver damage in an experimental model induced carbohydrate tetrachloride (CCl4), and the study of the regenerative capacity of the diseased liver for 2 months after seeding toxin. Methods. Obtaining models of liver cirrhosis in rats of Wistar 3 and 8 months of age, and was carried out by intraperitoneal injection of 30% animal oil solution of CCl4 (0.001 ml/kg) 1 day a week. The dynamics of the disease studied biochemical (measurement of ALT, AST), histological and radiological (SPL) methods. Results. By intraperitoneal injection of experimental mouse 1 times a week for 4 months, 30% carbon tetrachloride solution in sunflower oil at the rate of 1 l per 1 g was formed liver fibrosis caused by cytotoxic membrane-action poison. Characteristic of liver fibrosis were: violation of lobular structures alternate with layers of connective tissue, the presence of areas of necrosis parenchymal organ, a significant expansion of portal hypertension and portal vein diameter. During natural pathomorphism liver fibrosis in two months after seeding showed signs characteristic of deep liver fibrosis, but the percentage of damaged tissue visually reduced. This is also evidenced liver ultrasound. Significantly decreased performance ALT and AST 1.95 times to 1.46 times. Decreased liver weight 1.35 times and 2.44 times in the spleen. Thymus weight conversely increased 0.95 times. Conclusions. Thus, the presence of regenerative processes in the liver, as a result of which she maintains the balance indicates the need for monitoring and control of processes occurring in it, which will improve the results of treatment of the disease and its complications.

Key words: hepatic necrosis, fibrosis, regeneration, mouse.