Nucleus-nucleolus features of certain species of the Pinaceae family

  • Yu. O. Bilonozhko Institute of Food Biotechnology and Genomics, Nat. Acad. of Sci. of Ukraine, Ukraine, 04123, Kyiv, Оsypovskoho str., 2A
  • I. I. Korshikov Donetsk Botanical Garden of NAS of Ukraine, Ukraine, 50089, Kryvyi Rih, Marshak str., 50; Kryvyi Rih Botanical Garden of NAS of Ukraine, Ukraine, 50089, Kryvyi Rih, Marshak str., 50
Keywords: nuclear-nuclear indicators, interspecific diversity, conifers, Pinaceae


Aim: The aim of the study was to establish the interspecific features of the functioning of the nucleolus-forming organizer on the example of 6 species of the family Pinaceae. Methods. The study involved samples of different species of conifers that grow within the natural range and conditions of introduction. Silver nitric acid staining was used to visualize the nucleoli. Quantitative and dimensional features of the nucleus and nucleoli in the interphase cells of seedlings were studied, and the values of the nucleus-nucleolus ratio were calculated. Results. Interspecific polymorphism in the number and size of nucleoli, nuclei and nucleus-nucleolus ratio in six species and forms of the Pinaceae L. family was revealed. Conclusions. The studied species of conifers obviously have interspecific differentiation by nucleus-nucleolus parameters. The studied characteristics are influenced not only by the species, age and condition of the plant, but also by the natural and climatic conditions in which the population exists.


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