Assessment of mutagenic activity of toxic waste polygon (Kalush) soil’s chemical pollution by the level of cytogenetic disorders in Triticum aestivum L.
Aim. To study the frequency and spectrum of cytogenetic disorders in Triticum aestivum L. with prolonged action on soil xenobiotic seeds of toxic waste storage areas and to explore the level of their mutagenic activity in comparison with the action of NMS’s moderate and high concentrations. Methods. Cytogenetic disorders induced by soil’s chemical contamination of toxic waste landfill of Dombrovsky Quarry dump (Kalush city) and influence of NMS’s moderate and high concentrations were determined in the cells of the meristem root using the ana-thelophase method. Results. Soil’s chemical contamination of the studied objects exhibits high mutagenic activity, which exceeds the control level by 1.8–3.8 times and does not yield to the mutagenic activity of NMS’s moderate concentrations. The high frequency of chromosomal aberrations at the low concentration of hexachlorobenzene in the soil of the Dombrovsky Quarry dumps is a consequence of its complex interaction with natural mineral compounds of the mountain chemical raw materials. Conclusions. The increasing of the spectrum of cytogenetic disorders induced by chemical soil contamination the proportion of bridges and the chromosomal rings testify radiomimetic properties of the mutagenic factors. The increasing of the number of cells with multiple aberrations indicates a high genotoxicity of hexachlorobenzene and the risk of serious genetic consequences in the case of falling it’s into the environment.
Keywords: Triticum aestivum L., toxic waste, mitosis disorders, chromosomal aberrations, mutagenic activity.
Al-Naber H.M.F., Slenzak A., Stranadko N., Tykhyy V., Sulukhia T., Lvovsky K., Fan Q., Ahmed K. Ukraine country environmental analysis. The World Bank, Washington, 2016. 107 p. doi: 10.1596/24971.
Morgun V.V., Yakymchuk R.A. Cytogenetic anomalies of winter wheat cells, induced by chemical contamination of the territory of Kalush industrial district. Regulatory Mechanisms in Biosystems. 2018. Vol. 9, No. 3. P. 446–452. doi: 10.15421/021867
Rozhko M.M, Bilec’ka E.M., Shmatkov G.G., Erstenjuk G.M., Kryzhanivs’ka A.Ie., Onyshchenko S.V., Rud’ko G.I., Samojlik M.S., Semchuk Ja.M., Solovjov V.V. Development and implementation of a system for reducing the man-caused load on the territory and population of ecologically crisis areas. Ekolohiia i pryrodokorystuvannia. 2014. No. 18. Р. 97–110. [in Ukrainian]
Lysychenko G., Weber R., Kovach V., Gertsiuk M., Watson A., Krasnova I. Threats to water resources from hexachlorobenzene waste at Kalush City (Ukraine) – a review of the risks and the remediation options. Environmental Science and Pollution Research. 2015. Vol. 22, No. 19. P. 14391–14404. doi: 10.1007/s11356-015-5184-1.
Yakymchuk R.A., Valyuk V.F. Soil mutagenic activity in hazardous waste site of Kalush City (Western Ukraine). Ukrainian Journal of Ecology. 2018. Vol. 8, No. 1. P. 880–886. doi: 10.15421/2018_288.
Morgun V.V., Yakymchuk R.A. Genetic consequences of the Chornobyl NPP. Kyiv: Lohos, 2010. 400 p. [in Ukrainian]
Altwaty N.H., El-Sayed O.E., Aly N.A.H., Baeshen M.N., Baeshen N.A. Molecular and cytogenetic assessment of Dip-terygium glaucum genotoxicity. Anais da Academia Brasileira de Ciências. 2016. Vol. 88, No. 1. P. 623–634. doi: 10.1590/0001-3765201620150208.
Morgun V.V., Yakymchuk R.A., Azizov I.V. Рeculiarities of the mechanisms of spontaneous, and іnduced by ionizing radiation and chemical factors mutagenesis. Fiziologiya rasteniy i genetika. 2019. Vol. 51, No. 6. P. 463–481. doi: org/10.15407/frg2019.06.463.
Yakymchuk R.A. Genetic consequences of the contamination of the environment with natural and technogenic muta-genic factors. Kyiv: Lohos, 2019. 379 p. [in Ukrainian]
Pausheva Z.P. Workshop on plant cytology. Moscow: Agropromizdat, 1988. 271 p. [in Russian]
Lakin G.F. Biometric. Moscow: Vysshaja shkola, 1990. 352 p. [in Russian]