Genetic diversity of ukrainian wheat landraces Triticum aestivum L. at gliadin-coding loci
Aim. The aim of the study was to investigate genetic variability of gliadin-coding loci Gli-A1, Gli-B1, Gli-D1 in Ukrainian winter common wheat landraces and obsolete varieties. Methods. APAG electrophoresis of gliadin was used to identify genotypes at Gli-1 loci. Results. Diversity at the Gli-1 loci of 41 winter common wheat landraces, including local varieties Krymka, Banatka, Girka, Theyka, and 13 obsolete winter common wheat varieties, were studied. A total 31 gliadin alleles (including new 7 alleles) were revealed. There are 11 alleles at the Gli-A1 loci, 14 - at the Gli-B1, 6 -at the Gli-D1. Differences in frequencies of gliadin alleles were established. Conclusions. The high level of allelic variation at the Gli-1 loci was observed in Ukrainian winter common wheat landraces. Predominant alleles were revealed: Gli-A1m (46,8 %), Gli-A1d (21 %), Gli-B1b (47,1 %), Gli-D1g (45,8 %), Gli-D1a (33,3 %), Gli-D1j (13,9 %). In genotype of modern Ukrainian winter common wheat are present only 3 of them - Gli-B1b, Gli-D1g, Gli-D1j, which positively relate to dough quality.
Keywords: Triticum aestivum L., winter wheat, landraces, gliadin , alleles , polymorphism
Zhukovskiy P.M. Wheat in the SSSR. Moskva-Leningrad: Sel'khozgiz, 1957. 632p. [in Russian]
Vavilov N.I. Nauchnye osnovy selektsii pshenitsy. Moskva-Leningrad, 1935. 244 р. [in Russian]
Barash S.I. Old-local winter wheat of the Ukraine. Bulletin of applied botany, of genetics and plant breeding. 1975. vol. 55, N 3. P. 81-93 [in Russian]
Sozinov A.A., Poperelya F.A. Genetic classification of prolamins and its use for plant breeding. Ann. Technol. Agric. 1980. Vol. 29. P. 229–245.
Sozinov A.A. Polymorphism of protein and its importance in genetics and breeding. Moskva: Nauka, 1985. 272p. [in Russian]
Poperelya F. A. Polіmorfіsm of gliadin and his connection with grain quality, yield capacity and adaptivity winter common wheat varieties. Selektsiia, semenovodstvo i intensivnaia tekhnologiia vozdelіvaniia ozimoy pshenitsі. Moskva: Agropromizdat, 1989. S.138-150 [in Russian]
Sobko T.A., Poperelya F.A. The frequency of alleles of gliadin-coding loci in common winter wheat cultivars. Visnyk silskogospod. naukі. 1986. V. 5. P. 84–87 [in Ukrainian]
Chernakov K.M., Metakovsky E.V. Diversity of Gliadin-Coding Locus Allelic Variants and Evaluation of Genetic Similarity of Common Wheat Varieties from Different Breeding Centers. Genetika. 1994. V. 30. N4. P. 509–517 [in Russian]
Poperelya F.A. Three main genetic systems of the grain quality in winter common wheat. Realization of potential possibilities of varieties and hybrids of the Selection-Genetic Institute in conditions of Ukraine. Zbirnyk naukovykh prats SGI. 1996. P.117-132 [in Ukrainian]
Sobko T.A., Sozinov A.A. Analysis of the genotypic structure of common wheat cultivars licensed for growing in Ukrainne using genetics markers. Tsitologiya i genetika. 1999. V.33. No. 5. P.30-41 [in Russian]
Kozub N.A., Sozinov I.A., Sobko T.A., Kolyuchii V.T., Kuptsov S.V., Sozinov A.A. Variation at storage protein loci in winter common wheat cultivars of the Central Forest-Steppe of Ukraine. Cytol. Genet. 2009. V. 43, N 1. P. 55–62. doi: 10.3103/S0095452709010101
Catalogue of gene symbols. Gene catalogue, 2013. Retrieved from: htpp://www.shigen.nig.ac.jp/wheat/komugi/genes/download.jspMacGene
Metakovsky E.V. Gliadin allele identification in common wheat. II. Catalogue of gliadin alleles in common wheat. J.Genet. and Breed. 1991. V45. P.325-344.
Metakovsky E.V., Melnik V., Rodriguez-Quijano M., Upelniek V., Carrillo J.M. A catalog of gliadin alleles: Polymorphism of 20th-century common wheat germplasm. The Crop Journal. 2018. No. 6. P. 628-641. doi: 10.1016/j.cj.2018.02.003.
Sozinov A., Sozinov I., Kozub N., Sobko T. Stable gene associations in breeding and evolution of grasses. Evolutionary theory and processes: modern perspectives. Papers in Honor of Eviatar Nevo. Wasser, S.P. (ed.). Kluwer Academic Publishers, 1999. P. 97–113. doi: 10.1007/978-94-011-4830-6_7
Sobko T.A., Sozinov, A.A.. Genetical control of morphological characters in the ear and interrelations of allele variability of the marker loci of chromosomes 1A and 1B of common winter wheat. Tsitol. Genet. 1993. vol. 27, No. 5. P. 15–22. [in Russian]
Poperelya, F.A., Bito, M., and Sozinov, A.A. Association of blocks of gliadin components with survival of plants, their productivity, spike colour and flour quality in F2 hybrids from the cross of the varieties Bezostaya 1 and Crvena Zvezda. Dokl. VASKhNIL. 1980. Vol. 4. P. 4–7. [in Russian]
Pogna N.E., Metakovsky E.V., Radealli R., Raineri F., Dachkevitch T. Recombination mаpping of Gli-5, a new gliadin-coding locus on chromosomes 1A and 1B in common wheat . Theor. Appl. Genet. 1993. Vol. 87. P. 113-121. doi: 10.1007/BF00223754
Kozub N.A., Sozinov I.A., Karelov A.V., Blume Ya.B., Sozinov A.A. Diversity of Ukrainian winter common wheat varie-ties with respect to storage protein loci and molecular markers for disease resistance genes. Tsitologiya i genetika. 2017. V. 51. No. 2. P.59-73 [in Ukrainian] doi: 10.3103/S0095452717020050