Productivity of winter wheat plants with the additional copy of the ornithine-δ-aminotransferase gene under water deficit conditions
Aim. The evaluation of the productivity components of wheat biotech plants with the additional copy of the ornithine-δ-aminotransferase (oat) gene under water stress conditions. Methods. Field and laboratory approaches for studying the parameters of the crop structure. Results. A comparative analysis of productivity components of control plants and forms, obtained via Agrobacterium-mediated transformation were made. During plants cultivation under water deficit the genotype differences among variants were detected. Conclusions. It was showed that under osmotic stress pressure biotech plants (T2) of wheat winter genotypes Favoritka, Dostatok, Volodarka demonstrated better indices of crop structure compared with control plants. The differences between T2 Zolotocolosa and control plants were not essential. Biotechnological plants with the additional copy of the oat gene under poor water supply conditions were higher, had better developed root systems and formed the increased number of additional (lateral) shoots.
Keywords: Triticum aestivum L., biotechnological plants, ornithine-δ-aminotransferase gene, productivity components.
Morgun V.V., Kirisii D.A. Prospects and modern strategies for improving the physiological characteristics of wheat to increase its productivity. Physiology and Biochemistry of Cultivated Plants. 2012. Vol. 44, No 6. P. 463–483. [in Ukrainian]
Rybalka A.I. Wheat quality and its improving. Kyiv: Logos, 2011. 496 p. [in Ukrainian]
Morgun V.V. Problems of genetics and plant breeding in Ukraine at the turn of the millennium. Physiology and Biochemistry of Cultivated Plants. 2001. Vol. 33, № 5. P. 452–455. [in Ukrainian]
Mykhalska S.I., Sergeeva L.E, Matveyeva A.Yu., Kobernik N.I., Kochetov A.V., Tishchenko O.M., Morgun V.V. The free proline elevated levels of osmotolerant transgenic corn plants with dsRNA suppressor proline dehydrogenase gene. Plant Physiology and Genetics. 2014. Vol. 46, No 6. P. 482–489. [in Ukrainian]
Komisarenko A.G., Mykhalska S.I. The free proline levels in transgenic sunflower (Helianthus annuus L.) T3 plants with double-stranded proline dehydrogenase gene RNA-suppressor. Factors in experimental evolution of organisms. Kyiv: Logos, 2017. Vol. 20. P. 211–214. [in Ukrainian]
Morgun B.V., Tishchenko E.N. Molecular biotechnology to improve the resistance of cultivated cereals to osmotic stress. Kyiv: Logos, 2014. 221 p. [in Russian]
Mykhalska S.I., Komisarenko A.G., Kurсhii V.M., Tishchenko O.M. Agrobacterium-mediated in planta genetic transformation of winter wheat (Triticum aestivum L.). Factors in experimental evolution of organisms. Kyiv: Logos, 2018. Vol. 22. P. 293–298. [in Ukrainian]
Stránská J., Tylichová M., Kopecný D., Snégaroff J., Sebela, M. Biochemical characterization of pea ornithine-δ-aminotransferase: substrate specificity and inhibition by di- and polyamines. Biochimie. 2010. Vol. 92. Р. 940–948. doi: 10.1016/j.biochi.2010.03.026.
Gerasimova S.V., Kolodazhnaya Y.S., Titov S.E., Romanova A.V., Koval V.S., Kochetov A.V., Shumny V.K. Tobacco trans-formants expressing kDNA the ornithine amino transferase gene Medicago truncatulall. Genetics. 2010. Vol. 46, № 7. P. 1000–1003. [in Russian]
Roosens N.H., Bitar F.A., Loenders K., Angenon G., Jacobs M. Overexpression of ornthine-8-aminotransferase increases proline biosynthesis and confers osmotolerance in transgenic plants. Mol. Breed. 2002. Vol. 9. P. 73–80.
Likhochvor V.V. Structure of winter wheat crop. Lviv: Ukrainski tekhnologii, 1999. 200 p. [in Ukrainian]
Dospekhov B.A. Methods of field experiment. Moscow: Agropromizdat, 1985. 351 p.[in Russian]
Dubrovna O.V., Chugunkova T.V., Bavol A.V., Lyalko I.I. Biotechnological and cytogenetic bases for the creation of plants resistant to stress. Kyiv: Logos, 2012. 428 p. [in Ukrainian]
Mykhalska S.I., Komisarenko A.G., Kurсhii V.M., Obtaining biotech plants with increased resistance to stress. Climate change and agriculture: challenges for science and educatson: Book of abstracts of international scientific and practical conference with the support of the FAO. Kyiv, 2018. P. 453–457. [in Ukrainian]
Honcharuk O.M., Dubrovna O.V. Analysis of resistence osmotic transgenic wheat plants, carring the gene ornithine amіnotransferase. Factors in experimental evolution of organisms. Kyiv: Logos, 2017. Vol. 20. P. 173–178. [in Ukrainian]