Xerotolerant strain of Penicillium chrysogenum MF18_10 isolated from the damaged walls of Saint Sophia’s Cathedral, Kyiv

  • V. A. Lutsenko
  • L.V. Polishchuk
  • J. Hong
  • M.A. Fomina


Aim. The aim of this work is to determine the taxonomic position of the fungal strain MF18_10 isolated from the damaged wall with medieval painting of St. Sophia’s Cathedral (Kyiv, Ukraine). Methods. The fungus was isolated on selective medium for xerophilic fungi (70% sucrose Czapek agar). Мacro- and micro-morphological phenotypic characterization was carried out using light and scanning electron microscopy. Molecular-biological identification was performed using nucleotide sequences of the ITS-fragment. Results. On the basis of phenotypical characteristics of the fungal isolate MF18_10, it was established its affiliation to the genus Penicillium. ITS analysis revealed that the isolate belongs to the species P. chrysogenum demonstrating 100% identity with other 78 P. chrysogenum strains in GenBank database including the type strains NR_077145 and AY373902, also sharing several distinct differences in substitutions, deletions and insertions within this group. Conclusions. The isolated xerotolerant fungus was identified as P. chrysogenum, the typical representative of the indoor environments and dust, and also common for mycobiota of damaged historic-cultural artifacts. The differences in the analyzed P. chrysogenum ITS primary structures did not correlate with the source of isolation.

Keywords: Penicillium chrysogenum, xero-tolerant fungi, ITS, scanning electron microscopy.


Chen A., Hubka V., Frisvad J., Visagie C., Houbraken J., Meijer M. et al. Polyphasic taxonomy of Aspergillus section Asper-gillus (formerly Eurotium), and its occurrence in indoor environments and food. Studies in Mycology. 2017. Vol. 88. P. 37–135. doi: 10.1016/j.simyco.2017.07.001.

Liu Z., Zhang Y., Zhang F., Hu C., Liu G., Pan J. Microbial Community Analyses of the Deteriorated Storeroom Objects in the Tianjin Museum Using Culture-Independent and Culture-Dependent Approaches. Frontiers in Microbiology. 2018. Vol. 9. P. 802. doi: 10.3389/fmicb.2018.00802.

Unković N., Ljaljević Grbić M., Subakov-Simić G., Stupar M., Vukojević J., Jelikić A. et al. Biodeteriogenic and toxigenic agents on 17th century mural paintings and façade of the old church of the Holy Ascension (Veliki Krčimir, Serbia). Indoor and Built Environment. 2015. Vol. 25 (5). P. 826–837. doi: 10.1177/1420326X15587178.

Sklenář F., Jurjević Ž., Zalar P., Frisvad J. Visagie C., Kolařík M. et al. Phylogeny of xerophilic aspergilli (subgenus Aspergil-lus) and taxonomic revision of section Restricti. Studies in Mycology. 2017. Vol. 88. P. 161–236. doi: 10.1016/j.simyco.2017.09.002.

Tanney J., Visagie C., Yilmaz N., Seifert K. Aspergillus subgenus Polypaecilum from the built environment. Studies in Myco-logy. 2017. Vol. 88. P. 237–267. doi: 10.1016/j.simyco.2017.11.001.

Schoch C., Seifert K., Huhndorf S., Robert V., Spouge J., Levesque C. et al. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences. 2012. Vol. 109 (16). P. 6241–6246. doi: 10.1073/pnas.1117018109.

Houbraken J., Frisvad J., Seifert K., Overy D. Tuthill D., Valdez J. et al. New penicillin-producing Penicillium species and an overview of section Chrysogena. Persoonia – Molecular Phylogeny and Evolution of Fungi. 2012. Vol. 29 (1). P. 78–100. doi: 10.3767/003158512X660571.

Mueller G.M., Bills G.F., Foster M.S. Biodiversity of fungi. Inventory and monitoring methods. Burlington (USA): Elsevier Academic Press, 2006. 777 p.

Koval E.Z., Rudenko A.V., Goncharuk V.V., Voloshchuk N.M. Penicillia in the environment. Kyiv: Naukova Dumka, 2014. Part 1, 436 p., Part 2. 386 p. [in Ukrainian

Polishchuk L.V., Tkachenko K.S. Fomina M.A. Characteristics of ITS-fragment of rDNA of Rhodotorula mucilaginosa isola-ted from gastrointestinal tract of centenarian. Factors in Experimental Evolution of Organisms. 2017. Vol. 21. P. 284–287.

Fomina M.A., Polishchuk L.V., Tkachenko K.S., Hong J.W., Zelena L.B., Ianieva O.D., Pidgorskyi V.S. Complex identifica-tion of red yeast isolate from gastrointestinal tract of Hucul long-liver (Carpathians, Ukraine). Mikrobiolohichnyi zhurnal. 2016. Vol. 78 (5). P. 2–11.

Hong J., Fomina M., Gadd G. F-RISA fungal clones as potential bioindicators of organic and metal contamination in soil. Journal of Applied Microbiology. 2010. Vol. 109 (2). P. 415–430. doi: 10.1111/j.1365-2672.2009.04665.x.

An K. Kiyuna T., Kigawa R., Sano C., Miura S, Sugiyama J. The identity of Penicillium sp. 1, a major contaminant of the stone chambers in the Takamatsuzuka and Kitora Tumuli in Japan, is Penicillium paneum. Antonie van Leeuwenhoek. 2009. Vol. 96 (4). P. 579–592. doi: 10.1007/s10482-009-9373-0.

Coronado-Ruiz C., Avendaño R., Escudero-Leyva E., Conejo-Barboza G., Chaverri P., Chavarría M. Two new cellulolytic fungal species isolated from a 19th-century art collection. Scientific Reports. 2018. Vol. 8 (1). P. 7492. doi: 10.1038/s41598-018-24934-7.