Genetic variability of the storage protein loci in the european winter common wheat varieties licensed for growing in Ukraine

  • T. O. Sobko
  • G. M. Lisova
  • L. V. Sirant


Aim. The aim of the study was to investigate genetic variability of storage protein loci in European common winter wheat varieties licensed for growing in Ukraine in 2018 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the high-molecular-weight (HMW) glutenin loci Glu-1 and gliadin loci Gli-1. Results. Genotypes at the gliadin loci Gli-A1, Gli-B1, Gli-D1 and HMW glutenin loci Glu-A1, Glu-B1, Glu-D1 were identified in 48 winter common wheat varieties. A total of twenty gliadin alleles (including 10 at the Gli-A1, 4 at the Gli-B1, 6 at the Gli-D1 loci) and eleven HMW-GS alleles (including 3 at the Glu-A1, 5 at the Glu-B1, 3 at the Glu-D1 loci) were revealed. Differences in frequencies of alleles at storage protein loci were established. Conclusions. The high level of allelic variation was observed at the Gli-1 and Glu-1 loci in European common winter wheat varieties. Predominant alleles were revealed: Gli-A1о, Gli-A1а, Gli-A1f, Gli-B1f, Gli-D1b, Gli-D1d, Glu-А1c, Glu-А1a, Glu-B1с, Glu-B1d, Glu-D1d, Glu-D1a. Differences in frequencies of alleles at the storage protein loci between European and Ukrainian common winter wheat varieties were found.

Keywords: Triticum aestivum L., winter wheat, storage protein, alleles.


Sozinov A.A., Poperelya F.A. Genetic classification of prolamins and its use for plant breeding. Ann. Technol. Agric. 1980. Vol. 29. P. 229–245.

Sozinov A.A. Polymorphism of protein and its importance in genetics and plant breeding. M.: Nauka, 1985. 272 p. [in Rus-sian]

Catalogue of gene symbols. Gene catalogue, 2013. URL: htpp:// MacGene (Last accessed: 15.04.2019).

Sobko T.A., Poperelya F.A. The frequency of alleles of gliadin-coding loci in common winter wheat cultivars. Visnyk Sil-skogospod. naukі. 1986. Vol. 5. P. 84–87. [in Ukrainian]

Metakovsky E.V. Gliadin allele identification in common wheat. II. Catalogue of gliadin alleles in common wheat. J.Genet. and Breed. 1991. Vol. 45. P. 325–344.

Payne P.I., Lawrence G.J. Catalogue of alleles for the complex geneloci,Glu-A1, Glu-B1, and Glu-D1 which code for high-mole- cular-weight subunits of glutenin in hexaploid wheat. Cereal Res.Commun. 1983. Vol. 11, No. 1. P. 29–34.

Poperelya F.A. Three main genetic systems of the grain quality in winter common wheat. Realization of potential possibilities of varieties and hybrids of the Selection-Genetic Institute in conditions of Ukraine: zbirnyk naukovykh prats SHI. 1996. P. 117–132. [in Ukrainian]

Sobko T.O., Sozinov O.O. Characteristics of the Ukrainian winter common wheat varieties at the storage protein loci. Genetics and breeding in Ukrainian on the border of Millennium. Kyiv: Logos, 2001. Vol. 1. P. 151–159. [in Ukrainian]

Blagodarova O.M., Lytvynenko M.A., Golub Ye.A. Gene geography of alleles at gliadin- and glutenincoding loci of Ukrainian winter common wheat varieties and their associations with agronomical traits. Collection of Scientific papers of the Institute of Breeding and Genetics. 2004. Vol. 46, No. 6. P. 124–138. [in Ukrainian]

Kozub N.A., Sozinov I.A., Sobko T.A., Kolyuchii V.T., Kuptsov S.V., Sozinov A.A. Variation at storage protein loci in winter common wheat cultivars of the Central Forest-Steppe of Ukraine. Cytol. Genet. 2009. Vol. 43, No. 1. P. 55–62.

Wrigley, C.W., Bekes, F., Cavanagh, C.R., and Bushuk, W. The Gluten Composition of Wheat Varieties and Genotypes. 2006. URL: Pages/gliadin.aspx (Last accessed: 15.04.2019).

Kozub N.A., Sozinov I.A., Karelov A.V., Blume Ya.B., Sozinov A.A. Diversity of Ukrainian winter common wheat varieties with respect to storage protein loci and molecular markers for disease resistance genes. Tsitologiya i genetika. 2017. Vol. 51, №. 2. P. 59–73. [in Ukrainian] doi: 10.3103/S0095452717020050.

State register of plant varieties suitable for dissemination in Ukraine in 2018. [in Ukrainian] URL: (Last accessed: 15.04.2019).

Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970. Vol. 227. P. 680–685.

Metakovsky E.V., Melnik V., Rodriguez-Quijano M., Upelniek V., Carrillo J.M. A catalog of gliadin alleles: Polymorphism of 20th-century common wheat germplasm. The Crop Journal. 2018. No. 6. P. 628–641. doi: 10.1016/j.cj.2018.02.003.

Sobko T.A., Sozinov A.A. Analysis of the genotypic structure of common wheat cultivars licensed for growing in Ukrainne using genetics markers. Tsitologiya i genetika. 1999. Vol. 33, No 5. P. 30–41. [in Russian]

Metakovsky E.V., Branlard G. Genetic diversity of French common wheat germplasm studied using gliadin alleles. Theor. Appl. Genet. 1998. Vol. 96. P. 209–218.

Cerny J., Sasek A., Zummermann G., Selbert L. An evolution of Bavarian and Low Austrian new varieties of winter wheat by means of genetic protein markers of baking quality. Scientia Agric. Bohemoslov, 1992. No. 2. P. 113–123.

Sobko T.O., Sirant L.V., Lisova G.M. Genetic diversity of spring common wheat varieties at storage protein loci. Factors in experimental evolution of organisms. 2018. Vol. 23. P. 334–339. [in Ukrainian]

Poperelya F.O., Blagodarova O.M. Genetics of grain quality of first Ukrainian genotypes of superstrong wheat. Tsitol. Genet. 1998. Vol. 32, No. 6. P. 11–19. [in Ukrainian]

Panchenko I.A., Rabinovich S.V., Parhomenko R.G., Shevchenko Z.V. High-molecular-weight glutenin subunit composition of cultivars and breeding lines of hard red winter wheat from different geographic regions. Annual wheat newsletter. Kansas State University, 1996. Vol. 42. P. 212–216.

Chernakov K.M., Metakovsky E.V. Diversity of Gliadin-Coding Locus Allelic Variants and Evaluation of Genetic Similarity f Common Wheat Varieties from Different Breeding Centers. Genetika. 1994. Vol. 30, No. 4. P. 509–517. [in Russian]

Sozinov A., Sozinov I., Kozub N., Sobko T. Stable gene associations in breeding and evolution of grasses. Evolutionary theory and processes: modern perspectives. Papers in Honor of Eviatar Nevo. Wasser, S.P. (ed.). Kluwer Academic Publishers, 1999. P. 97–113.