Biochemical characteristics of soybean varieties under viral infection and different growth conditions

  • O. O. Моlodchenkova
  • L. T. Mishchenko
  • T. V. Kartuzova
  • L. Ya. Bezkrovnaya
  • О. B. Likhota
  • G. D. Lavrova
  • Е. Sh. Мursakaev


Aim. The aim of the work was to investigate of particularities of biochemical composition of seed and plant vegetative mass of different soybean varieties at the action of viral infection and agroclimatic conditions. Меthods. Standard and adapted methods of biochemical analysis were used for laboratory research. Isolation of glycinin and β-conglycinin was carried out using method developed in the laboratory (Pat #42181). Results. It was established that infection by Soybean mosaic virus (SKP-16 and SGP-17 isolates) causes changes in the biochemical characteristics (content of protein, main storage protein fractions (glycinin and β-conglycinin), fat, carbohydrates, isoflavones, activity of lectin, lipoxygenase, trypsin inhibitor) in the infected seeds that depend on the soybean variety, the infection degree of the plants and the conditions of cultivation. The determination of the relative moisture content, proline content, and lectin activity in the leaves of the 2 upper layers of soybean plants, which differed on the level of drought-tolerance in the phases of flowering, bob formation and filling of beans showed that contents of all studied biochemical characteristics significantly increased in the drought-tolerant soybean varieties as compared with not drought-tolerant ones in the phase of filling of beans. Conclusions. The obtained results can be used for development of the methods of soybean varieties selection with high seed quality and complex resistance (to the cultivation conditions and viral infection) and will be recommended for implementation in breeding and agricultural practices.Keywords: soybean, plant breeding, SМV, drought, biochemical characteristics.


Irvin M.E., Schultz G.A. Soybean mosaic virus. FAO. Plant Prot. Bull. 1981. Vol. 29. P. 41–55.

Campos R.E., Bejerman N., Nome C., Laguna I., Rodriguer P.P. Bean Yellow mosaic virus in soybean from Argentina. J. Phytopatholog. 2014. Vol. 162. P. 222–325.

Mishchenko L.T., Dunich A.A., Shevchenko T.P., Budzanivska I.G., Polischuk V.P., Andriychuk O.M., Molchanets O.V., Antipov I.O. Detection of soybean mosaic virus in some left-bank forest-steppe regions of Ukraine. The Microbiologic Jour-nal. 2017. Vol. 79, № 3. P. 3–14.

Li T., Didorenko S., Orazbaeva U., Spankulova Z., Tashkenova A., Birimzshanova Z. Biochemical indexes of soybean drought tolerance. Eurasian Journal of Applied Biotechnology. 2013. No 3. P. 35–40. [in Russian]

Babosha A.V. Inducible lectins and plant resistance to pathogenic organisms and abiotic stresses. Biochemistry. 2008. Vol. 73, No 7. P. 1007–1022. [in Russian]

Mishchenko L.T. Virus diseases of winter wheat. Kyiv: Phytosocicenter, 2009. 352 p. [in Ukrainian]

Martinez M., Rubio-Somora I., Carbonenro P., Diaz I. A cathepsin B-like cystein protease gene from Hordeum Vulgare (gene CatB) induced by GA in aleurone cells is under circadian control in leaves. J. Exp. Bot. 2003. Vol. 5. P. 951–959.

Tarchevsky I.A. Signalling systems of plant cells. M.: Nauka, 2002. 292 p. [in Russian]

Parr A.J., Bolwell G.P. Phenols in the plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenols content or profile. Journal of the Science of Food and Agriculture. 2000. Vol. 80. P. 985–1012.

Szabodos L., Savoure A. Proline: a multifunctional amino acid. Trends Plant Sci. 2009. Vol. 15, № 2. P. 89–97.

Dospechov B.A. Field technique (with basis of statistical processing of research results). 5-th revised and enlarged edition. Моskow: Аgropromizdat, 1985. 351 p. [in Russian]

Crowther J.R. ELISA. Theory and practice. New York: Hamana Press, 1995. 223 p.

Sherepitko D.V., Budzanivska I.G., Polischuk V.P., Boyko A.L. Sequencing and phylogenetic analysis of Soybean mosaic virus isolated in Ukraine. 2011. Biopolymers and Cell. Vol. 27, № 6. P. 472–479. doi: 10.7124/bc.00011A.

Huelsenbeck J.P., Rannala B. Maximum likelihood estimation of phylogeny using stratigraphic data. Paleobiology. 1997. Vol. 23, № 2. P. 174–180.

Muhire B.M., Varsani A., Martin D.P. SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE. 2014. Vol. 9, № 9. e108277. doi: 10.1371/journal.pone.0108277.

Levitsky A.P. Extractive method of fat determination in plant primary produce using extractos of new type. Proceeding of scientific investigations of All Union PBI ‘Biochemical methods of breeding material research’. Odessa: All Union PBI, 1979. Vol. 15. P. 78–84. [in Russian]

Lucik M.F., Panasyuk E.N., Lucik A.D. Lectins. Lviv: Vuscha shkola, 1980. 150 p. [in Russian]

Budnitskaya E.V. Research of lipoxygenase activity of feeder greens using method of carotin oxidating. Biochemistry. 1955. Vol. 20, № 5. P. 615–621. [in Russian]

Vasyukova A.N. Study of total flavonoids content in the seed and seedlings of soybean. Agricaltural sciences and agro-industrial complex. 2013. No 4. P. 9–13. [in Russian]

Mishenko L., Dunich A., Mishenko I., Molodchenkova O. Molecular and biological properties of soybean mosaic virus and its influence on the yield and quality of soybean under climate change conditions. Agriculture and foresty. 2018. Vol. 64, No 4. P. 39–47.