Genetic analysis of oleic acid content in the oils of maize sugary-2 mutants

  • D. S. Tymchuk
  • G. S. Potapenko
  • N. F. Tymchuk
  • V. V. Muzhylko


Aim. Genetic analysis of oleate content in the carriers of maize mutation su2. Methods. Experiments were performed on a series of unrelated inbreeds – carriers of mutation su2 which were hybridized by the diallel and top – cross crossings. The analysis of the fatty acid composition of oil were carried out using a modified gas-chromatographic Peyskare method. Results. It was found, that su2 mutants were notable as having the increased content of oleate compared to the maize of usual type and this trait has a quantitative nature. The inbreeds – carriers of su2 mutation were significantly differed in the the effects of combining abilities for the oleate content. The inheritance of oleate was carried out by the type of incomplete dominance with the prevailing contribution of additive effects to the variance. Conclusions. In the sixth maize chromosome it is very likely that there is a spatial linkage of the su2 and oleate- encoding locus, the effect of which can be modified by the polygenic complex.

Keywords: Zea mays L., mutation su2, oleic acid, genetic analysis.


Warner K., Knowlton S. Frying quality and oxidative stability of high – oleic corn oils. J. Amer. Oil. Chem. Soc. 1997. Vol. 74. P. 1317–1322. doi: 10.1007/s11746-997-0063-7.

Pravst I. Oleic acid and its potential health effects. Oleic acid. Production, uses and potential health effects: monograph / L. Whelan Ed. New-York: Nova Sci. Publ. Inc., 2014. Cpt. 3. P. 35–54.

Lee E.A. Maize for oil. Oil crops: monograph / J. Vollmann, I. Raican Eds. Dordrecht – Heidelberg – London – New-York: Springer Sci., 2009. Chpt. 17. P. 493–506. doi: 10.1007/978-0-387-77594-4.

Whight A. A gene conditioning high oleic maize oil, OCL1. Maydica. 1995. Vol. 40. P. 85–88.

Alrefai R., Berke T.G., Rocheford T.R. Quantitative trait locus analysis of fatty acid concentrations in maize. Genome. 1995. Vol. 38. P. 894–901. doi: 10.1139/g95-118.

Motto M., Balconi C., Hartings H., Rossi V. Gene discovery for improvement of kernel quality - related traits in maize. Ge-netika. 2010. Vol. 42. P. 23–56. doi: 10.2298/GENSR1001023M.

Belo A., Zheng P., Luck S., Shen B., Meyer D.J., Li B., Tingey S., Rafalski A. Whole genome scan detects an allelic variant of fad2 associated with increased oleic acid levels in maize. Mol. Gen. Genomics. 2008. Vol. 279. P. 1–10. doi: 10.1007/s00438-007-0289-y.

Neuffer M.G., Coe E.H., Wessler S.R. Mutants of maize: monograph. Cold Spring Harbor: Cold Spring Harbor Lab. Press, 1997. 468 p.

Tymchuk D.S., Potapenko G.S., Tymchuk N.F, Muzhilko V.V. Genetic analysis of oleic acid content in the oil of maize on the basis of mutation sugary-1. Factory eksperymental’noi evoliutsii orhanizmiv: zbirnyk naukovykh prats’ / V.A. Kunakh (holov. red.) [ta in.]. Kyiv: Ukrains’ke tovarystvo henetykiv i selektsioneriv im. M.I. Vavilova, 2018. V. 23. P. 137–142. [in Ukrain-ian]

Wassom J.J., Mikkelineni V., Bohn M.O., Rocheford T.R. QTL for fatty acid composition of maize kernel oil in Illinois High Oil x B73 backcross-derived lines. Crop Sci. 2008. Vol.48. P. 69–78. doi: 10.2135/cropsci2007.04.0208.

Val D.L., Schwartz S.H., Kerns M.R., Dierkman J. Development of high oil trait in maize. Molecular genetic approaches to maize improvement: monograph / A.L. Kriz, B.A. Larkins Eds. Berlin – Heidelberg: Springer – Verlag, 2009. P. 303–324. doi: 10.1007/978-3-540-68922-5-1.

Yang X., Guo Y., Yan J., Zhang J., Song T., Rocheford T., Li J.-S. Major and minor QTL and epistasis contribute to fatty acid compositions and oil concentration in high-oil maize. Theor.Appl.Genet. 2010. Vol. 120. P. 665–678. doi: 10.1007/s00122-009-1184-1.

Zheng P., Allen W.B., Roesler K., Williams M.E., Zhang S., Li J., Glassman K., Ranch J., Nubel D., Solawetz W., Bhat-tramakki D., Llaca V., Deschamps S., Zhong G.Y., Tarczynski M.C., Shen B. A phenylalanine in DGAT is a key determinant of oil content and composition in maize. Nat.Genet. 2008. Vol. 40. P. 367–372. doi: 10.1038/ng.85.

Dospekhov B.A. Technique of field experiment. Moskva. Agropromizdat, 1985. 351 p. [in Russian]

Prokhorova M.I. Biochemical research methods. Leningrad: Khymiia, 1982. 272 p. [in Russian]

Lakyn G.F. Biometrics. Moskva: Vysshaia shkola, 1973. 343 p. [in Russian]

Litun P.P., Proskurnin N.V. Genetics of quantitative traits: genetic crossings and genetic analysis. Kiev: UMVO, 1992. 96 s. [in Russian]

Zhang X., Colleoni C., Ratushna V., Sirghie-Colleoni M., James M., Myers A. Molecular characterization demonstrates that the Zea mays gene sugary2 codes for the starch synthase isoform SSIIa. Plant Mol. Biol. 2004. Vol. 54. P. 865–879. doi: 10.1007/s11103-004-0312-1.

Shanklin J., Cahoon E.B. Desaturation and related modifications of fatty acids. Annu Rev Plant Physiol Plant Mol Biol. 1998. Vol. 49. P. 611–641. doi: 10.1146 /annurev. arplant.49.1.611.