Nucleotide sequences of tRNA-methonine genes of Streptomyces globisporus 1912-2, identified in silico

  • L. V. Polishchuk Institute of Microbiology and Virology of NAS of Ukraine, Ukraine, 03680, Kyiv, Akademika Zabolotnoho str., 154


Aim of this work was to identify nucleotide sequences of tRNAMet of S. globisporus 1912-2. Methods. Resources of server NCBI (programs BLAST: blast, discontiguous megablast and databases: “Genome”, “Nucleotide”) were used for in silico analysis of library of S. grlobisporus 1912-2 contigs. Results. Nucleotide sequences of 4 genes of tRNAMet of S. globisporus 1912-2 were determined in silico. Molecules of tRNA of the II type were translated from tRNAMet gene (Contig № 21 (936–1008 bp)) and the molecules of tRNAfMet genes (Contigs № 299 (1713–1787 bp), № 255 (5941–6015 bp)). Conclusions. 4 genes of transfer RNAs-methionine were identified in silico in S. globisporus 1912-2 genome. Two genes from them coded tRNAfMet molecules. The nucleotide sequences of all tRNAMet genes were identified.

Keywords: gene, tRNA, methionine, nucleotide sequence, Streptomyces.


Lehninger A. Principles of biochemistry. Vol. 3. Moskva: Mir, 1985. 367 p.

Gualerzi C.O., Pon C.L. Initiation of mRNA translation in bacteria: structural and dynamic aspects. Cell. Mol. Life. Sci. 2015, Vol. 72(22). P. 4341-4367. doi: 10.1007/s00018-015-2010-3

Kuchino Y., Yamamoto I., Nishimura S. Nucleotide sequence of Streptomyces griseus initiator tRNA. Nucleic. Acids. Res. 1982. Vol. 10(21). P. 6671-6684. doi: 10.1093/nar/10.21.6671

Gamulin V., Soll D. The initiator tRNA genes from Streptomyces rimosus. Nucleic. Acids. Res. 1987. Vol. 15(16). P. 6747. doi: 10.1093/nar/15.16.6747

Nakamoto U. Evolution and the universality of the mechanism of initiation of protein synthesis. Gene. 2009. Vol. 432(1-2). P. 1-6. doi: 10.1016/j.gene.2008.11.001

Samhita L., Shetty S., Varshney U. Unconventional initiator tRNAs sustain Escherichia coli. Proc. Natl. Acad. Sci. USA. 2012. Vol. 109(32). P. 13058-13063. doi: 10.1073/pnas.1207868109

Samhita L., Nanjundiah V., Varshney U. How many initiator tRNA genes do Escherichia coli need? J. Bacteriol. 2014. Vol. 196(14). P. 2607-2615. doi: 10.1128/JB.01620-14

Plohl M., Gamulin V. Five transfer RNA genes lacking CCA termini are clustered in the chromosome of Streptomyces rimosus. Mol. Gen. Genet. 1990. Vol. 222(1). P. 129-134. doi: 10.1007/BF00283033