The content of polyphenolic compounds in the Arabidopsis thaliana knockout mutant Cat2 under salt stress

  • N. O. Didenko Yuri Fedkovych National University of Chernivtsi, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine
  • I. M. Buzduga Yuri Fedkovych National University of Chernivtsi, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine
  • R. A. Volkov Yuri Fedkovych National University of Chernivtsi, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine
  • I. I. Panchuk Yuri Fedkovych National University of Chernivtsi, Kotsiubynski str. 2, 58012 Chernivtsi, Ukraine


Aim. In plants, the defense response to abiotic stress includes induction of stress proteins and increase in content of protective metabolites. To date, the role of specific isoforms of antioxidant enzymes in stress responses and their relation to low-molecular weight protective compounds are still not clarified. To study this question the content of polyphenolic compounds (PPС) was evaluated under salt stress in Arabidopsis thaliana wild-type (WT) and in catalase 2 (Cat2) knockout mutant plants. Methods. PPС content in different variants of treatment with sodium chloride was measured. Results. It was shown that under optimal cultivation conditions the content of PPS in leaves of cat2 mutants is higher than in WT leaves. However, cultivation of isolated shoots in nutrient medium resulted in a faster depletion of the PPС pool in the cat2 line. Also, short-term salt stress results in equal depletion of the PPС pool in both, WT and cat2. Conclusions. The increase of PPС content in cat2 leaves is a manifestation of metabolic alterations that aim to compensate the reduced catalase activity.

Keywords: multigenic family, knockout mutants, polyphenolic compounds, sodium chloride, Arabidopsis thaliana.


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