Analysis of protein content and level of 7S and 11S globulins, their ratio in the soybean seed of F6 - F8 hybrid lines
Aims. The goal of research was to study a character of changes and relationship between level of 7S and 11S globulins, their ratio and protein content in the soybean seed of F6-F8 hybrid lines and their parental forms of different genetic origin in the connection of protein quality breeding. Methods. 7S and 11S globulins were isolated by method, which was developed in the Laboratory of Plant Biochemistry (Patent # 42181). Results. The features of 7S and 11S globulins content and their ratio in the soybean seed of F6 -F8 hybrid lines and their parental forms of different genetic origin were identified. It was established, that varieties and hybrid lines are characterized by considerable distinctions of 7S and 11S globulins contents and their ratio in the soybean protein. A connection between 7S and 11S globulins levels and protein content in the seed of soybean F6- F8 hybrid lines wasn’t established. Conclusions. It was elucidated that the growth conditions influence on the protein content, 11S and 7S globulins level, their ratio and relationship between these parameters in the soybean seeds of F6-F8 hybrid lines and their parental forms of different genetic origin, so that have to be introduced in the selection program of soybean genotypes of food direction soybean breeding as these parameters connected with the food nutritional quality.
Key words: Glicine max L., protein, 11S globulin, 7S globulin.
Klimenko V. G. Belki semian bobovykh rasteniy. Kishinev: Shtiitsa, 1978. 344 p.
Adamovskaia V. G., Molodchenkova O. O., Sichkar V. I., Tsiselskaia L. I. Sortovye osobennosti belkovo-fermentnogo kompleksa i tekhnologicheskie kharakteristiki sortov soi. Khranenie i pererabotka zerna. 2003. No 10 (52). P. 27-52.
Adamovskaia V. G., Molodchenkova O.O., Sychkar V. Y., Tsyselskaia L.Y. Biokhimichni osoblyvosti bilkovoho kompleksu nasinnia soi ta vykorystannia yoho u vyrobnytstvi. Tematychnyy naukovyy zbirnyk “Selektsiia i nacinnytstvo”. Kharkiv, 2005. No 90. P. 162-175.
Yaklich R. W. β-Conglycinin and Glycinin in High-Protein Soybean Seeds. J. Agric. Food Chem. 2001. Vol. 49(2). P.729-735. doi: 10.1021/jf001110s
Harada K., Toyokawa Y., Kitamura K. Genetic analysis of the most acidic 11S globulin subunit and related characters in soybean seeds. Japanese Journal of Breeding. 1983. Vol. 33. P. 23-30. doi: 10.1270/jsbbs1951.33.23
Moraes R.M.A. de, Jose I. C., Ramos F. G., Barros E. G. de, Moreira M. A. Caracterização bioquímica de linhagens de soja com alto teor de proteína. Pesq. agropec. bras. 2006. Vol. 41. P. 725-729. doi: 10.1590/S0100-204X2006000500002
Takahashi M., Hajika M., Matsunaga R. et al. Breeding soybean variety lacking β-conglycinin by introduction of Scg gene from wild soybean. In International soybean processing and utilization conference 3. Tsukuba proceedings. Tsukuba “The Japanese Society for food and science and technology”. 2000. P. 45-46.
Adamovskaia V. G., Molodchenkova O. O., Sichkar V. I., Lavrova G. D., Kartuzova T. V., Bezkrovnaia L. Y. Soderzhanie 7S i 11S globulinov i ikh vzaimosviaz s belkovistostiu semian gibridnykh populiatsiy soi. Sbornik nauchnykh trudov SGI. 2014. No. 23 (63). P. 37-42.
Adamovska V. G., Molodchenkova O. O., Sichkar V. I., Tsiselska L.Y., Sagaydak T. V. Patent na korisnu model No 42181. Sposib doboru soi. 25.06.2009.
Carrao-Panizzi M. C., Kwanyuen P., Erhan Z., Lopes I. O. N. Genetic variation and environmental effects on the beta-conglycinin and glycinin content in Brazilian soybean cultures. Pesq. agropec. bras. 2008. Vol. 43(9). P. 1105-1114. doi: 10.1590/S0100-204X2008000900002
Galao O.F., Teixeira A.I., Moreira M.A., Carrao-Panizzi M.C., Visentainer J.V. Variation in genetic and environmental effects of beta-conglycinin (7S) and glycinin (11S) protein fractions conventional and GM soybean cultivar grown in Southern Brazil. Semina: Ciencias Agrarias. 2013. V. 34(2). P. 683-692. doi: 10.5433/1679-0359.2013v34n2p683