Peculiarities of chromosomal variability in cultured tissues of Deschampsia antarctica Desv. plants with different chromosome numbers

  • V. A. Kunakh Institute of Molecular Biology and Genetics of NAS of Ukraine, Ukraine, 03680, Kyiv, Akademika Zabolotnoho str., 150
  • D. O. Navrotska Institute of Molecular Biology and Genetics of NAS of Ukraine, Ukraine, 03680, Kyiv, Akademika Zabolotnoho str., 150
  • M. O. Twardovska Institute of Molecular Biology and Genetics of NAS of Ukraine, Ukraine, 03680, Kyiv, Akademika Zabolotnoho str., 150
  • I. O. Andreev Institute of Molecular Biology and Genetics of NAS of Ukraine, Ukraine, 03680, Kyiv, Akademika Zabolotnoho str., 150


Aim. To clarify the details of chromosome variation in calli derived from D. antarctica plants in the initial passages of the culture in vitro. Methods. Induction of callus from root explants of plants, which were grown from seeds, and consequent subcultivation of tissue culture. Cytogenetic analysis of squashed slides stained by acetic-orcein and counting the number of chromosomes in mitotic metaphase plates. Results. There were analyzed the cultured tissues derived from D. antarctica plants with different chromosome numbers: diploid plants (2n=26), mixoploid plant with B-chromosomes (2n=26+1-3В), and mixoploid plant with near-triploid modal class (2n=36, 38). Analysis of callus tissues of all plants at 2-4 passages revealed mixoploidy, presence of polyploid and aneuploid cells. The modal class in all studied calli was composed of diploid and aneuploid cells with near-diploid chromosome number. The cytogenetic structure of cell population of cultured tissues was found to vary with characteristics of the karyotype of donor plant. The largest range of variation in the number of chromosomes (from 18 to 63 chromosomes) was found in tissue culture of diploid plant (2n=26) from the Galindez Island, and the highest frequencies of polyploid (47 %) and aneuploid cells were in the culture of mixoploid plant with near-triploid modal class from the Big Yalour Island. Conclusions. In different D. antarctica cultured tissues at the early stages of the culture, the modal class was composed of diploid cells and cells with near-diploid chromosome number irrespective of karyotype of donor plant (diploid, mixoploid poliploid).

Key words: Deschampsia antarctica Desv., plant tissue culture, chromosomal variability in vitro, mixoploidy.


Zimmerman L. Somatic embryogenesis: a model for early development in higher plants. Plant Cell. 1993. No 5. P.1411–1423. doi: 10.1105/tpc.5.10.1411

Kunakh V.A. Biotechnology of medicinal plants. Genetic, physiological and biochemical basis. Kyiv: Logos, 2005. P. 730 (in Ukrainian).

Kunakh V.A. Ontogenetic plasticity of the genome as a basis for plant adaptivity. Zhebrakovsky readings. III. Transformation of genomes. Minsk: Pravo i ekonomika, 2011. P. 56 (in Russian).

McClintock B. The states of a gene locus in maize. Carnegie Institute of Washington Yearbook. 1968. No 66. P. 20–28.

McClintock B. The significance of responses of the genome to challenge. Science, 1984. No 226. P. 792–801. doi: 10.1126/science.15739260

Madlung A., Comai L. The effect of stress on genome regulation and structure. Annals of Botany. 2004. No 94. P. 481–495. doi: 10.1093/aob/mch172

Neelakandan A. K., Wang K. Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications. Plant Cell Reports. 2012. No 31. P. 597–620. doi: 10.1007/s00299-011-1202-z

Bravo L.A., Ullloa N., Zuniga G.E., Casanova A., Corcuera L.J., Alberdi M. Cold resistance in Antarctic angiosperms. Physiologia Plantarum. 2001. No 111. P. 55–65. doi: 10.1034/j.1399-3054.2001.1110108.x

John U.P., Polotnianka R.M., Sivakumaran K.A., et al. Ice recrystallization inhibition proteins (IRIPs) and freeze tolerance in the cryophilic Antarctic hair grass Deschampsia antarctica E. Desv. Plant, Cell and Environment. 2009. No 32. P. 336–348. doi: 10.1111/j.1365-3040.2008.01925.x

Parnikoza I., Kozeretska I., Kunakh V. Vascular plants of the Maritime Antarctic: origin and adaptation. American Journal of Plant Sciences, 2011. Vol. 2(3). P. 381–395. doi: 10.4236/ajps.2011.23044

Chew O., Lelean S., John U.P., Spangenberg G.C. Cold acclimation induces rapid and dynamic changes in freeze tolerance mechanisms in the cryophile Deschampsia antarctica E. Desv. Plant, Cell and Environment. 2012. No 35. P. 829–837. doi: 10.1111/j.1365-3040.2011.02456.x

Navrotska D.O., Twardovska M.O., Andreev I.O., Zahrychuk O.M., Parnikoza I.Yu., Drobyk N.M., Kunakh V.A. Chromosomal polymorphism of Deschampsia antarctica Desv. plants from Argentine Islands region (maritime Antarctic). Visn. Ukr. Tov. Genet. Sel. 2014. Vol. 12(2). P. 184–190 (in Ukrainian).

Navrotska D.O., Twardovska M.O., Andreev I.O., Parnikoza I.Y., Betekhtin A.A., Zahrychuk O.M., Kunakh V.A. New forms of chromosome polymorphism in Deschampsia antarctica Desv. from the Argentine islands of the Maritime Antarctic region. Ukrainian Antarctic Journal. 2014. No 13. P. 185–191.

Amosova A.V., Bolsheva N.L., Samatadze T.E. et. al. Molecular cytogenetic analysis of Deschampsia antarctica Desv. (Poaceae), Maritime Antarctic. PLOS ONE. 2015. Vol. 10(9): e0138878. doi: 10.1371/journal.pone.0138878

Zagrychuk O.M., Herts A.I., Drobyk N.M., Kunakh V.A. Callus formation and regeneration of Deschampsia antarctica Desv. (Poaceae) in culture in vitro. Biotechnologia Acta. 2013. Vol. 6(6). P. 77–86 (in Ukrainian).

Zahrychuk O.M., Drobyk N.M., Kozeretska I.A., Parnikoza I.Yu. , Kunakh V.A. Introduction in culture in vitro of Deschampsia аntarctica Desv. (Poaceae) from two regions of Maritime Antarctica. Ukrainian Antarctic Journal, 2011/2012. No 10–11. P. 289–295 (in Ukrainian).

Gamborg O.L., Eveleigh D.E. Culture methods and detection of glucanases in cultures of wheat and barley. Canadian Journal of Biochemistry, 1968. Vol. 46(5). P. 417–421. doi: 10.1139/o68-063

Kunakh V.A., Levenko B.A. Modification of squash technique for studying chromosomes in plant tissue culture cells. Tsitologia i genetika, 1975. Vol. 9(1). P. 56–58 (in Russian).

Plohynskyy N.A. Biometrics. 2-nd edition. Moscow: MGU, 1970. P. 367.

Kunakh V.А. Evolution of cell populations in vitro: peculiarities, driving forces, mechanisms and consequences. Biopolymers and Cell, 2013. Vol. 29(4). P. 295–310. doi: 10.7124/bc.000824.

Zosymovych V.P., Levenko B.A., Kunakh V.A., Yurkova G.N. Cytogenetic study of tomato callus tissues from plants of different ploidy. In: Culture of plant cells. Kyiv: Naukova Dymka, 1978. P. 97–104 (in Russian).

Fras A., Maluszynska J. Regeneration of diploid and tetraploid plants of Arabidopsis thaliana via callus. Acta Biologica Cracoviensia. Series Botanica. 2003. Vol. 45(2). P. 145–152.