The interaction of verprolin WIRE with the adapter proteins family intersectin

  • S. V. Kropyvko Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150
  • A. V. Rynditch Institute of Molecular Biology and Genetics of Natl. Acad. Sci. of Ukraine, Ukraine, 03143, Kyiv, Akad. Zabolotnogo str., 150


Aim. WIRE is a scafold protein that regulates actin cytoskeleton rearrangements and the formation of actin enriched membrane processes responsible for invasion and migration. ITSN1 and ITSN2 are representatives of the family of intersectins who participate in the reorganization of the actin cytoskeleton, as well as in other processes, such as endo/enzocytosis, cellular signal transduction, etc. As these proteins participate in the same processes, we checked their interaction with each other. Methods. Protein-protein interactions were identified using the GST pull-down method. Results. We showed that the SH3 domains of ITSN1 and ITSN2 interact with WIRE, and found that while WIRE is in a complex with endogenous actin. Conclusions. ITSN1 and ITSN2 interact with WIRE, which is located in a complex with endogenous actin.
Keywords: WIRE, ITSN1 and ITSN2, actin.


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