Use of DNA markers for studying the genetic polymorphism of the bread wheat (T. aestivum Desf.) genotypes of Azerbaijan

  • E. S. Hajiyev
  • Z. I. Akparov
  • R. T. Aliyev
  • S. M. Babayeva
  • V. I. Izzatullayeva
  • M. A. Abbasov
  • A. D. Mamedova

Abstract

Aim. The study was devoted to molecular-genetic evaluation of 110 bread wheat genotypes collected from different regions of Azerbaijan. Methods. Molecular-genetic. Results. The mean polymorphism level for the studied genotypes of bread wheat, using 8 ISSR markers, was 72 %. An index of genetic diversity calculated for the studied collection of T. aestivum specie was equal to 0.91. Among the bread wheat accessions, the highest genetic diversity was established for botanical variety var. erythrospermum (GDI=0.90). Conclusions. Primers UBC 112, UBC 841, UBC 857 and UBC 873 were identified to be more effective in identification of bread wheat accessions. As a result of the GGE biplot analysis, a trait collection was created by selecting 20 bread wheat samples with broad genetic variation. А core collection of bread wheat was created, which coversthe most distant genotypes and represents the genetic diversity of all 110 accessions studied.

Keywords: Triticum aestivum Desf., genetic polymorphism, ISSR markers.

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